Leicester defender Ben Chillwell could be in line for a second senior England appearance after receiving a late call to the squad.
Chillwell made his debut last month when England faced Switzerland in an international friendly at Leicester’s King Power Stadium.
The left-back had initially been called up to the Under-21 squad but Manchester United defender Luke Shaw has been forced to pull out through injury.
“Luke Shaw has withdrawn from the England squad and is returning to Manchester United, meaning the in-form defender will miss the two Uefa Nations League matches against Croatia and Spain,” said a statement from Manchester United.
“The 23-year-old’s exit from the Three Lions’ camp was announced by The FA on Monday evening, and he will now return to the Aon Training Complex for treatment.”
Chillwell joins Leicester team-mates Harry Maguire and James Maddison in manager Gareth Southgate’s Three Lions squad for the clashes with Croatia and Spain.
England face World Cup runners-up Croatia on Friday and they are 2.75 to claim the win, while Maddison is available at 10.00 to score the first goal.
Croatia can be backed at 2.70 and a draw is 3.10. England are 15.00 to win the Nations League outright, Croatia can be backed at 29.00 and Spain are the current 3.75 favourites.
Southgate’s men have played one game in the tournament so far and were beaten 2-1 by Spain, while the Croats were humbled 6-0 by La Furia Roja in their Nations League opener.