England have lost the services of both Michael Carrick and Andros Townsend for their Euro 2016 qualifier against Slovenia at Wembley on Saturday.
Manchester United midfielder Carrick has been forced out of the game due to a groin problem, while Tottenham winger Townsend misses out because of an ankle problem.
No replacements have been called up by head coach Roy Hodgson, with both players also ruled out of the friendly clash with Scotland next Tuesday.
England go into the game against Slovenia with three wins out of three in Group E, having triumphed 2-0 in Switzerland, thrashed San Marino 5-0 and edged a 1-0 victory in Estonia last time out.
The Three Lions can be backed at 1.32 to make it four wins out of four by seeing off Slovenia and are 1.57 to keep a fourth consecutive clean sheet in qualifying.
The two sides have met on two previous occasions, with England winning both matches, the most recent coming at the World Cup finals in South Africa in 2010, when Jermain Defoe netted the only goal of the game.
The former Spurs striker was also on target in a 2-1 friendly win in September of the previous year.
Slovenia are currently second in the Group E, having taken six points from a possible nine to lie three points behind England. The Slovenes opened up with a 1-0 loss to Estonia before responding with a 1-0 victory over Switzerland and a 2-0 success in Lithuania.
Slovenia are hoping to qualify for just their second ever European Championship finals, having done so in 2000 when they failed to progress past the group stage.